Table 1 The oligonucleotide sequence of primers used for detecting expression of genes of interest, in the RT-PCR assay.
Oligonucleotide | Sequence | Amplified length | Annealing temperature |
|---|---|---|---|
PGM1–1718 | 5'-TCCGACTGAGCGGCACTGGGAGTGC-3' | 386bp | 63°C |
PGM1–2080 | 5'-TCCGACTGAGCGGCACTGGGAGTGC-3' | ||
hTR-F | 5'-CGCCGTGCTTTTGCT CC-3' | 316bp | 63°C |
hTR-R | 5'-ACTCGCTCCGTTCCTCTTCC-3' | ||
hTERT–13187 | 5'-CGG AAG AGT GTC TGG AGC AA-3' | 145bp | 67°C |
hTERT–15411 | '5'-TCC AGA CTC CGC TTC ATC C-3' |