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Figure 1 | International Seminars in Surgical Oncology

Figure 1

From: Evaluation of the Taguchi methods for the simultaneous assessment of the effects of multiple variables in the tumour microenvironment

Figure 1

Experimental runs 1–16 show the different survival rates of K562 cells as a result of culturing the cells in medium enriched by different combinations of the 4 cytokines (GMCSF, MCSF, IL-3, and GCSF). The maximum cytotoxicity of daunorubicin was observed as a result of the addition of 300 U/ml of MCSF, and 50 ng/ml of the other 3 cytokines (experimental run 3). The maximum survival of the cells was observed when the concentration of GMCSF in this mixture was reduced to 10 ng/ml (experimental run 16). Experiments 17 – 48 suggested that MCSF interacts with the 3 other factors to affect daunorubicin cytotoxicity. This could be seen by comparing the effect of the individual factors (runs 17 – 24) with the effects of the addition of two factors simultaneously. For example, experimental run 30 shows the concurrent effect of both MCSF (100 U/ml) and GMCSF (50 ng/ml) that resulted in a survival that was significantly higher than that caused by any of the two factors alone (runs 17, 18, 21 & 22). Experimental run 26 also represents the combined effects of MCSF (100 U/ml) and IL-3 (50 ng/ml), which resulted in a survival that was higher than the resulting survival of any of the two factors individually. Run 36; on the other hand, represents the increase in daunorubicin cytotoxicity as a result of the simultaneous addition of MCSF (300 U/ml) and GCSF (50 ng/ml). All these experimental runs were done in triplicate and repeated 3 times, the results are expressed as mean ± SE.

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