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Table 4 Taguchi method L8(27). This array accommodated 4 different factors (MCSF, IL-3, GMCSF, and GCSF) each at 2 different concentrations (see above). 8 experimental runs were carried out according to the combination of factors in the array, for example, in experimental run 1 the MTT assay was carried out after mixing the cells with 100 U/ml MCSF, 10 ng/ml IL-3, 10 ng/ml GMCSF, and 10 ng/ml GCSF. The interaction between MCSF and the other three factors (IL-3, GMCSF and GCSF) was studied in this array.

From: Evaluation of the Taguchi methods for the simultaneous assessment of the effects of multiple variables in the tumour microenvironment

  A MCSF B IL3 AxB C GMCSF AxC AxD D GCSF
1 1 1 1 1 1 1 1
2 1 1 1 2 2 2 2
3 1 2 2 1 1 2 2
4 1 2 2 2 2 1 1
5 2 1 2 1 1 1 2
6 2 1 2 2 2 2 1
7 2 2 1 1 1 2 1
8 2 2 1 2 2 1 2