Table 2 Primer sequences for exons 2, 3, & 13
From: Contribution of BRCA1 germline mutation in patients with sporadic breast cancer
BRCA1 | EXON | PRIMER Sequences(5'-to-3') | Product size | Tm(°C) | |
|---|---|---|---|---|---|
EXON2 | F | GGTTGTGATTAGTTCTTTGG | 458 | 51.3 | |
R | GTGTTGAAAAGGAGAGGAGT | 53.4 | |||
EXON3 | R | GAATGAAATGGAGTTGGATT | 381 | 55.81 | |
F | AGGATCGTATTCTCTGCTGT | 53.98 | |||
EXON13 | R | AGAACCAAGGCTCCATAAT | 476 | 54 | |
F | ATTGCATGAATGTGGTTAGA | 53.76 | |||